Carbon tetrachloride ccl 4induced hepatic damage in experimental sprague dawley rats. Carbon tetrachloride ccl 4 is well known to induce hepatotoxicity after being metabolized to trichloromethyl free radical ccl 3 by cyp2e1. Jayaraman, plant anatomy research centre, chennai, india. Pdf hepatoprotective effect of cleome gynandra on carbon. Protective effects of coumarin and coumarin derivatives against carbon tetrachlorideinduced acute hepatotoxicity in rats. Riboflavin attenuates carbon tetrachlorideinduced oxidative stress. This work characterizes the metabolism disorders of hepatotoxicity induced by ccl 4 in a wistar rat model with a single dosage of 1 mlkg. The carotenoid bixin present in annatto is an antioxidant that can protect cells and tissues against the deleterious effects of free radicals. Comparison of two protocols of carbon tetrachloride. Protective effect of cow urine distillate in three dose levels and standard drug silymarin 100 mgkg, p.
Protection against carbon tetrachlorideinduced hepatotoxicity by pretreating rats with the hemisuccinate esters of tocopherol and cholesterol. In this study we have evaluated its potential protective effect on hepatic injury and fibrosis induced by carbon tetrachloride ccl 4. Toxicity was induced by using 30% ccl 4 suspended in olive oil 1. Concomitant treatment of rats with a nonhepatotoxic dose of dichloromethane 6 mmolkg, ip significantly potentiated the hepatotoxicity of carbon tetrachloride 2 mmolkg, ip. Hepatoprotective activity of leptadenia reticulata stems against carbon tetrachlorideinduced hepatotoxicity in rats amit kumar nema 1, abhinav agarwal 2, varsha kashaw 1 1 pharmacognosy research laboratory, sagar institute of pharmaceutical sciences, sagar, madhya pradesh, india 2 pharmaceutics research laboratory, department of pharmaceutical sciences, dr.
Prevents carbon tetrachloride induced hepatotoxicity in rats article pdf available in international journal of applied research in natural products 22 june 2009 with 1,402 reads. Toxicity was determined by increases in serum sorbitol dehydrogenase and alanine aminotransferase activities measured 24 hr following the treatments. Hepatoprotective effects of phosphatidylserine liposomes on carbon tetrachloride. M w fariss, k f bryson, e e hylton, h r lippman, c h stubin, and.
The present study was conducted to investigate the hepatoprotective activity of hydroethanolic fruit extract of rosa canina r. The present study evaluated the protective effects of the flavonoidrich fraction from rhizomes of smilax glabra roxb. Potential effects of garlic oil and curcumin on carbon. Standardization of carbon tetrachlorideinduced hepatotoxicity in the rat. Carbon tetrachloride ccl 4 induced hepatotoxicity and nephrotoxicity model was used to investigate the possible mechanisms of fullerenol protection in spraguedawley rats in this study. Treatment of rats with carbon tetrachloride produced severe liver injury, as demonstrated by dramatic elevation of serum alt, ast. Rats were individually treated daily with 300 and 600 mgkg dose of either ethyl acetate, ethanol or aqueous extracts of a. We studied the role and regulation of nos in carbon tetrachloride ccl 4induced hepatotoxicity in newborn rats. Introduction liver is the most important organ, which plays a pivotal role in regularity various physiological process.
Cleome gynandra has been used widely in indian indigenous system. Research article open access protective effect of bixin. Protective effect of ricinus communisleaves extract on carbon tetrachloride induced hepatotoxicity in albino rats evan sabina princea, poorna parameswarib, rasool mahaboob khanc, aschool of biotechnology, chemical and biomedical engineering, vit university, vellore 632 014, india. Dichloromethane potentiation of carbon tetrachloride. Examples of these natural products are garlic oil and curcumin that are characterized by their high antioxidant activity. Don gentianaceae on carbon tetrachlorideinduced liver disorders were investigated. The results of the present study showed the hepatoprotective effects of ps against ccl 4. Toxicity was induced by using 30% ccl4 suspended in olive oil 1. Carbon tetrachloride ccl 4 is a wellknown model compound for inducing chemical hepatic injury. Carbon tetrachlorideinduced hepatotoxicity in pregnant. This study was planned to investigate the protective effect of vitamin c on chronic and acute models of carbon tetrachloride ccl 4induced oxidative stress and changes of. View enhanced pdf access article on wiley online library html view download pdf for. Pdf standardization of carbon tetrachlorideinduced.
In the present study the hepatoprotective effect of methonolic extract of c. In particular, for carbon tetrachloride poisoning, at a time when liver triglycerides are increasing rapidly, there is no increase in flux of fatty acids through the plasma compartment. Amaranthus lividus, antioxidant enzymes, carbon tetrachloride, hepatotoxicity. Carbon tetrachlorideinduced hepatotoxicity in rats. Protective effect of bixin on carbon tetrachlorideinduced. Protection of hepatic damage caused by carbon tetrachloride administration was observed by recording sgot, sgpt, alp and bln levels because they have been reported to be. Many herbal natural products have succeeded in reducing the toxicity of many toxins, such as that caused by carbon tetrachloride.
In the present study, the hepatotoxicity induced by a single oral dose 2,000 mgkg of ccl 4 was compared between pregnant gestation days gd and 19 or postpartum postpartum days ppd 1, and 27 and nonpregnant rats. Evaluation of silymarin and or ginger effect on induced hepatotoxicity by carbon tetrachloride in male albino rats hassan h. Wistar albino rats weighing around 180200g were used. Groups df were administered orally the cholestin in diet for 1%, 2%, 3%, group g served as positive control and was given silymarin in diet for 12%. Modulatory effect of mangifera indica against carbon. Riboflavin reduces carbon tetrachlorideinduced increase in tnf riboflavin, a component of flavoproteins plays an important role in hepatoprotection. Murat bilgin h, atmaca m, deniz obay b, ozekinci s, tasdemir e, ketani a. Hepatoprotective and antioxidant activity of bombax ceiba. Effect of cow urine distillate on liver function was studied in vivo in rats intoxicated with carbon tetrachloride ccl 4.
Nonalcoholic fatty liver sensitizes rats to carbon tetrachloride hepatotoxicity shashikirandonthamsetty,1 vishakhas. Protective effects of pergularia daemia roots against. Antioxidant potential of xylopia aethiopica adewale o. Nacetyl cysteine, hepatotoxicity, carbon tetrachloride carbon tetrachloride is commonly used as a model to evaluate hepatotoxicity 1. Hepatoprotective effect of rosa canina fruit extract. The protective effect of garlic oil, curcumin and their combination against ccl4 hepatotoxicity wa. Biochanin a protects against acute carbon tetrachloride. A sevenday long continuous collection of urine was performed in male rats in this experiment. Modulatory potentials of the aqueous stem bark extract of mangifera indica on carbon tetrachlorideinduced hepatotoxicity in rats. In this study, we evaluated the protective effect of bixin on liver damage induced by carbon tetrachloride ccl4 in. Carbon tetrachlorideinduced hepatotoxicity in rat is reversed by treatment with ribo. The aim of this study was to investigate the possible protective effects of echinacoside, one of the phenylethanoids isolated from the stems of cistanches salsa, a chinese herbal medicine, on the free radical damage of liver caused by carbon tetrachloride in rats.
Evaluation of silymarin and or ginger effect on induced. Protective effects of fullerenol on carbon tetrachloride. The role of urtica dioica and nigella sativa in the. Rutin is a polyphenolic natural flavonoid which possesses antioxidant and anticancer activity. Effects of yam peel extract against carbon tetrachloride. Wistar albino rats were administered 250 and 500 mgkg body weight extract orally for 10 consecutive days. Hepatoprotective agents could prevent tissue damage and reduce morbidity and mortality rates. Quercetin and kaempferol were active components in the juice. Modulatory effect of mangifera indica against carbon tetrachloride induced kidney damage in rats. Hepatotoxicity was induced in rats by carbon tetrachloride ccl 4 treatment. O, onikanni a, sabiu saheed abstract the present study was carried out to evaluate the hepatoprotective effects of aqueous extract of.
Alharbia, muzaffar iqbalb, sheikh fayaz ahmada a department of pharmacology and toxicology, college of pharmacy, king saud university, riyadh, saudi arabia b department of pharmaceutical chemistry, college of pharmacy, king saud. Attenuation of carbon tetrachlorideinduced hepatotoxicity. Pdf the aqueous seed extract of carica papaya linn. Hepatoprotective effects of phosphatidylserine liposomes. The present study was conducted to evaluate the hepatoprotective effects of an aqueous leaf extract of coccinia grandis on carbon tetrachloride ccl4 induced liver damage in albino rats. Posttranslational activation of endothelial nitric oxide. In the present study the hepatoprotective effect of rutin was evaluated against carbon tetrachloride ccl 4induced liver injuries in rats.
Protective effect of ricinus communis leaves extract on. In vivo protective effect of rosmarinus officinalis oil against carbon tetrachloride ccl4induced hepatotoxicity in rats. Biochanin a protects against acute carbon tetrachlorideinduced hepatotoxicity in rats randa m. Effect of whey protein against fluvastatin and carbon tetrachlorideinduced hepatotoxicity in rats ezz eldin s. Despite being a cardinal experimental model, the induction of cirrhosis in rats by repeated exposure to carbon tetrachloride ccl4 has low reproducibility. Glutathione plays an important role in the detoxification of most toxic agents. An important biologicallyrelevant property of fullerenol is its ability to quench free radicals. The most recent work in this field has inaugurated a third revolution in the study of. Vitamin a deficiency potentiates carbon tetrachlorideinduced liver fibrosis in rats. Protective effect of secoisolariciresinol diglycoside in carbon tetrachloride induced hepatotoxicity in rats sadiq s mareai 1, khalid m naji2, kavishankar gawli 3 and j rajesha 3 1department of chemistry, biochemistry division, university of thamar, thamar, yemen 2department of chemistry, university of sanaa, sanaa, yemen 3department of biochemistry, yuvarajas college, university of. Potentiation of carbon tetrachloride hepatotoxicity and lethality in type 2 diabetic rats. The liver is an important organ for its ability to transform xenobiotics, making the liver tissue a prime target for toxic substances.
Histological effects of carotenoid on carbon tetrachloride. The hepatoprotective and antioxidant effect of an ethanolic extract of rocket eruca sativa l. Carbon tetrachloride hepatotoxicity pharmacological. A total of seventy five rats were divided into five 5 groups of. Phytochemical screening of this plant revealed the presence flavanoids, saponins, terpenoids and tannis. Pdf carbon tetrachloride ccl4 induced hepatotoxicity. Eer, on liver injury induced by carbon tetrachloride ccl 4 was investigated. Nonalcoholic fatty liver sensitizes rats to carbon. This is mainly due to the effective antioxidant potential of nacetyl cysteine. Hepatoprotective activity of leptadenia reticulata stems. Materials and methods plant material roots of pergularia daemia were collected from the maruthamalai hills, coimbatore, india in november 2006 and identified by p. Hesperidin alleviates zinc oxide nanoparticle induced hepatotoxicity and oxidative stress. The active constituent profile in cape gooseberry physalis peruviana l.
Pessayre d, cobert b, descatoire v, degott c, babany g, funckbrentano c, delaforge m, larrey d 1982 hepatotoxicity of trichloroethylenecarbon tetrachloride mixtures in rats. Mehendale1 this study tested whether hepatic steatosis sensitizes liver to toxicantinduced injury and. Sgf on carbon tetrachloride ccl4induced hepatotoxicity in rats. Carbon tetrachlorideinduced hepatotoxicity and nephrotoxicity in rats. A study to produce ideal hepatotoxicity rats models using varying concentrations of carbon tetrachloride ccl 4 was carried out. Hepatotoxic interaction between carbon tetrachloride and. Patel, moses samuel rajan, shenoy ashok, shabaraya a. Protective effects of echinacoside on carbon tetrachloride. The present study was designed to evaluate the possible hepatoprotective effects of riboflavin against carbon tetrachloride ccl 4 induced hepatic injury in rats. Ideal hepatotoxicity in rats using carbon tetrachloride ccl4. Liver is a vital organ for the detoxification of toxic substances present in the body and hepatic injury is associated with excessive exposure to toxicants. Hepatoprotective effect of adenema hyssopifolium g. M zoology department, faculty of science, mansoura university, mansoura, egypt. Carbon tetrachloride induces hepatotoxicity via oxidative stress and inflammation.